LC-MS/MS based targeted metabolomics method for analysis of serum and cerebrospinal fluid

Szymon Plewa; Paweł Dereziński; Jolanta Florczak-Wyspiańska; Karolina Popławska-Domaszewicz; Wojciech Kozubski; Bartosz Sokół; Roman Jankowski; Jan Matysiak; Zenon J. Kokot

doi:10.20883/jms.2019.335

Authors

Szymon Plewa Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0002-9600-3980
Paweł Dereziński Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0002-6066-1260
Jolanta Florczak-Wyspiańska Department of Neurology, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0002-7131-8687
Karolina Popławska-Domaszewicz Department of Neurology, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0002-9154-389X
Wojciech Kozubski Department of Neurology, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0003-2777-261X
Bartosz Sokół Department of Neurosurgery, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0003-4814-2000
Roman Jankowski Department of Neurosurgery, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0002-2368-4578
Jan Matysiak Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0002-9993-1504
Zenon J. Kokot Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poland http://orcid.org/0000-0003-4950-9759

DOI:

https://doi.org/10.20883/jms.2019.335

Keywords:

liquid chromatography, tandem mass spectrometry, flow injection analysis

Abstract

Introduction. Recent instrumentation and software advancement enabled to develop new, high‑throughput targeted metabolomics methods for in‑depth exploration of metabolome in a quantitative manner.
Material and Methods. The presented targeted metabolomics approach allows to analyze both of serum and CSF in the same way, with identical sample preparation procedures. The analyses were carried out using high‑performance liquid chromatography system coupled to triple quadrupole tandem mass spectrometer with electrospray ion source (LC‑ESI‑QqQ‑MS/MS).
Results. The applied targeted metabolomics approach enabled to determine a wide panel of metabolites from different chemical classes of compounds including: acylcarnitines, amino acids and biogenic amines, glycerophospholipids, sphingolipids and sum of hexoses. Finally, 148 metabolites in serum and 57 in cerebrospinal fluid were determined.
Conclusions. Here we presented the results of successful implementation of the method of analysis of low‑molecular weight compounds in human serum and CSF using targeted metabolomics. The evaluation of selected groups of metabolites resulted in obtaining the mean concentrations of panel of metabolites in serum and CSF, which gives a valuable information about the metabolome of these matrices.

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