Estimation of Phagocytic Activity by Normal Human Peripheral Blood Mononuclear Cells on Various Oral Isolates of Candida Species: an in-vitro Study

PJ Sreelakshmi; Kumar KS Harish; Jacob Kuruvilla; Philip George Theckel

doi:10.20883/medical.e953

Authors

PJ Sreelakshmi Department of Medical Microbiology, School of Medical Education, Centre for Professional and Advanced Studies, Kottayam, Kerala, India
Kumar KS Harish Department of Medical Microbiology, School of Medical Education, Centre for Professional and Advanced Studies, Kottayam, Kerala, India https://orcid.org/0000-0002-0504-7794
Jacob Kuruvilla Department of Public Health Dentistry, Mar Baselios Dental College, Ernakulam, Kerala, India
Philip George Theckel Electron Microscopy Unit, Faculty of Medicine, Kuwait University, Kuwait

DOI:

https://doi.org/10.20883/medical.e953

Keywords:

phagocytic activity, candida species, respiratory burst, nitroblue tetrazolium test, antifungal susceptibility

Abstract

Polymorphonuclear neutrophils (PMN) and mononuclear phagocytes represent an important first line and effector function in controlling Candida infections. The study aims to determine the in-vitro phagocytic activity of human peripheral blood mononuclear cells against oral isolates of Candida species and its antifungal susceptibility. The study also evaluates the degree of respiratory burst activity of PBMCs. Phagocytic and lytic indices by PBMCs were determined for Candida spp. The respiratory burst activity was evaluated using a nitroblue tetrazolium test. Antifungal disc diffusion susceptibility testing was performed.

100 Candida were isolated, belonging to C. albicans, C. tropicalis, C. krusei and C. auris. The phagocytic and lytic indices of C. albicans were significant compared to the standard strain of C. albicans. C. tropicalis and C. krusei phagocytic index were significant, while the lytic index was insignificant compared to the standard strain. The inter-species comparison of both indices was not significant for the clinical isolates of Candida. A significant reduction in phagocytic activity was observed for clinical isolates of Candida spp. However, lytic activity was variable compared to the standard strain of C. albicans.

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