Bisphenol A modifies human spermatozoa motility in vitro
Keywords:bisphenol A, human spermatozoa, sperm cells motility
AbstractIntroduction. The decrease of men’s sperm quality was reported to be related to exposure to xenoestrogens. Bisphenol-A (BPA) is a synthetic xenoestrogen commonly present in our environment, for instance in food containers.
Aim. The aim of this study was to investigate the influence of BPA on human spermatozoa motility.
Material and methods. The effects on spermatozoa of BPA at final concentrations of 10–10, 10–8 and 10–6 mol/L were studied regarding to the following phenomena: (1) evaluation of sperm motility using computer-aided sperm analysis system providing four parameters: velocity straight linear VSL, cross beat frequency CBF, lateral head displacement LHD and homogeneity of progressive movement velocity HPMV, (2) spermatozoa vitality (propidium iodide staining), (3) phosphatidylserine membrane translocation (staining with annexin V conjugated with fluorescein) and (4) kinetics of intracellular free calcium ions changes (using Fluo-3).
Results. BPA caused a transient, significant increase of VSL and HPMV at 15 minutes after stimulation. One hour incubation of spermatozoa with BPA did not alter cells vitality nor stimulated phosphatidylserine membrane translocation, for all three concentrations. BPA in the final concentration of 10–6 mol/L initiated a rapid (observed after a few seconds), and transient (resolving after a few minutes) increase of intracellular free calcium ions concentration.
Conclusions. Human spermatozoa can be considered target cells for BPA. BPA significantly modified spermatozoa motility. BPA affected spermatozoa involving free calcium ions as second messenger.
Skakkebaek NE, Rajpert-De Meyts E, Buck Louis GM, Toppari J, Andersson AM, Eisenberg ML, Kold Jensen T, et al. Male reproductive disorders and fertility trends: Influences of environment and genetic susceptibility. Physiol Rev. 2016;96:55–97.
La Rocca C, Tait S, Guerranti C, Busani L, Ciardo F,Bergamasco B, Perra G, et al. Exposure to endocrine disruptors and nuclear receptors gene expression in infertile and fertile men from italian areas with different environmental features. Int J Environ Res Public Health. 2015;12:12426–45.
Watson CS, Jeng YJ, Guptarak J. Endocrine disruption via estrogen receptors that participate in nongenomic signaling pathways. J Steroid Biochem Mol Biol. 2011;127:44–50.
Rozati R, Reddy PP, Reddanna P, Mujtaba R. Role of environmental estrogens in the deterioration of male factor fertility. Fertil Steril. 2002;78:1187–94.
Rozati R, Reddy PP, Reddanna P, Mujtaba R. Xenoesterogens and male infertility: myth or reality? Asian J Androl. 2000;2:263–9.
World Health Organization, Food and Agriculture Organization of Unated Nations: Bisphenol A (BPA) Current state of knowledge and future actions by WHO and FAO. International Food Safety Authorities Network (INFOSAN), 2009 [dostęp: 26 września. 2013], dostępne:http://www.who.int/foodsafety/publications/fs_management/No_05_Bisphenol_A_Nov09_en.pdf.
EFSA. Scientific Opinion on the Risks to Public Health Related to the Presence of Bisphenol A (BPA) in Foodstuffs—Part I: Exposure Assessment. Available online: http://www.efsa.europa.eu/sites/default/files/scientific_output/files/main_documents/3978part1.pdf (accessed on 6 August. 2015).
Sui Y, Ai N, Park SH, Rios-Pilier J, Perkins JT, Welsh WJ, et al. Bisphenol A and its analogues activate human pregnane X receptor. Environ Health Perspect. 2012;120:399–405.
Tait S, Tassinari R, Maranghi F, Mantovani A. Bisphenol A affects placental layers morphology and angiogenesis during early pregnancy phase in mice. J Appl Toxicol. 2015;35:1278–91.
Aquila S, Sisci D, Gentile M, Middea E, Catalano S, Carpino A, Rago V. Estrogen receptor (ER) alpha and ER beta are both expressed in human ejaculated spermatozoa: evidence of their direct interaction with phosphatidylinositol-3-OH kinase/Akt pathway. J Clin Endocrinol Metab. 2004;89:1443–51.
Rago V, Siciliano L, Aquila S, Carpino A. Detection of estrogen receptors ER-alpha and ER-beta in human ejaculated immature spermatozoa with excess residual cytoplasm. Reprod Biol Endocrinol. 2006;4:1–6.
Ikezuki Y, Tsutsumi O, Takai Y, Kamei Y, Taketani Y. Determination of bisphenol A concentrations in human biological fluids reveals significant early prenatal exposure. Hum Reprod. 2002;17:2839–41.
Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J. Effect of estrogens on boar sperm capacitation in vitro. Reprod Biol Endocrinol. 2010;8:87.
Filannino A, Stout TAE, Gadella BM, Sostaric E, Pizzi F, Colenbrander B, et al. Dose-response effect of estrogenic mycotoxins (zearalenone, alpha- and beta-zearalenol) on motility, hyperactivation and the acrosome reaction of stallion sperm. Reprod Biol Endocrinol. 2011;9:134.
Fujinoki M, Takei GL, Kon HJ. Non-genomic regulation and disruption of spermatozoal in vitro hyperactivation by oviductal hormones. J Physiol Sci. 2015 Nov;1–6.
Jin W, Arai KY, Watanabe G, Suzuki AK, Takahashi S, Taya K. The stimulatory role of estrogen on sperm motility in the male golden hamster (Mesocricetus auratus). J Androl. 2005;26:478–84.
Hatef A, Alavi SM, Abdulfatah A, Fontaine P, Rodina M, Linhart O. Adverse effects of bisphenol A on reproductive physiology in male goldfish at environmentally relevant concentrations. Ecotoxicol Environ Saf. 2012;76:56–62.
De Flora S, Micale RT, a Maestra S, Izzotti A, D’Agostini F, Camoirano A, Davoli SA, et al. Upregulation of clusterin in prostate and DNA damage in spermatozoa from bisphenol A-treated rats and formation of DNA adducts in cultured human prostatic cells. Toxicol Sci. 2011;122:45–51.
Perobelli JE, Martinez MF, da Silva Franchi CA, Fernandez CD, de Camargo JL, Kempinas WG. Decreased sperm motility in rats orally exposed to single or mixed pesticide. J Toxicol Environ Heath. 2010;73:991–1002.
Mohamed EA, Park Y, Song W, Shin D, You Y, Ryu B, Pang M. Xenoestrogenic compounds promote capacitation and an acrosome reaction in porcine sperm. Theriogenology. 2011;75:1161–9.
Li DK, Zhou Z, Miao M, He Y, Wang J, Ferber J, Herrinton LJ, Gao E, Yuan W. Urine bisphenol-A (BPA) level in relation to semen quality. Fertil Steril. 2011;95:625–30.
Durkee TJ, Mueller M, Zinaman M. Identification of estrogen receptor protein and messengerribonucleic acid in human spermatozoa. Am J Obstet Gynecol. 1998;178:1288–97.
Solakidi S, Psarra AM, Nikolaropoulos S, Sekeris CE. Estrogen receptors alpha and beta (ERalpha and ERbeta) and androgen receptor (AR) in human sperm: localization of ERbeta and AR in mitochondria of the midpiece. Hum Reprod. 2005;20:3481–7.
Kotwicka M. Błonowa translokacja fosfatydyloseryny jako wykładnik biologicznych procesów zachodzących w plemnikach ludzkich. Wydaw. Nauk. Uniw. Med. im. K. Marcinkowskiego, Poznań. 2008.
Kotwicka M, Jendraszak M, Skibińska I, Jędrzejczak P, Pawelczyk L. Decreased motility of human spermatozoa presenting phosphatidylserine membrane translocation-cells selection with the swim-up technique. Hum Cell. 2013;26:28–34.
Kotwicka M, Filipiak K, Jedrzejczak P, Warchol JB. Caspase-3 activation and phosphatidylserine membrane translocation in human spermatozoa: is there a relationship? Reprod Biomed Online. 2008;16:657–63.
Baldi E, Luconi M, Muratori M, Marchiani S, Tamburrino L, Forti G. Nongenomic activation of spermatozoa by steroid hormones: facts and fictions. Mol Cell Endocrinol. 2009;308:39–46.
Espinosa F, López-González I, Munoz-Garay C, Felix R, De la Vega-Beltrán JL, Kopf GS,Visconti PE, Darszon A. Dual regulation of the T-type Ca2+ current by serum albumin and betaestradiol in mammalian spermatogenic cells. FEBS Lett. 2000;475:251–6.
Luconi M, Bonaccorsi L, Forti G, Baldi E. Effects of estrogenic compounds on human spermatozoa: evidence for interaction with a nongenomic receptor for estrogen on humansperm membrane. Mol Cell Endocrinol. 2001;178:39–45.
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Copyright (c) 2016 Malgorzata Kotwicka, Izabela Skibinska, Natalia Piworun, Magdalena Jendraszak, Malgorzata Chmielewska, Piotr Jedrzejczak
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