Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells

Authors

  • Hanna Szaefer Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poland https://orcid.org/0000-0001-5875-3425
  • Katarzyna Hadryś Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poland
  • Hanna Gajewska Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poland
  • Kinga Migdałek Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poland
  • Violetta Krajka-Kuźniak Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poland https://orcid.org/0000-0001-7275-0298

DOI:

https://doi.org/10.20883/medical.e805

Keywords:

Betanin, STAT3, STAT5, KAP1, HepG2 cells, THLE-2 cells

Abstract

Background. Several studies suggest that the pleiotropic properties of betanin may interfere with different signaling pathways. Our previous studies on human hepatocytes showed that betanin activated the nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway. To further understand the exact mechanism of action of betanin, we evaluated its effect on the levels of signal transducers and activators of transcription (STATs) and KRAB domain-associated protein 1 (KAP1) in hepatoma cells (HepG2) and normal human hepatocytes (THLE-2).

Material and methods. HepG2 and THLE-2 cells were treated with 2 or 10 µM betanin for 72 h. The levels of STAT3, STAT5a, STAT5b, and KAP1 proteins in cytosolic and nuclear fractions were assessed by Western blot.

Results. At a concentration of 10 μM, betanin significantly decreased the levels of STAT3, STAT5a, and STAT5b proteins in the nuclear fraction of HepG2 cells. On the other hand, no significant changes in the levels of STAT proteins were observed in THLE-2 cells. In HepG2 cells, betanin at both tested doses increased the level of KAP1. In contrast, in THLE-2 cells, betanin at a dose of 10 µM decreased the nuclear level of KAP1.

Conclusions. Betanin modulated the levels of STAT3, STAT5, and KAP1 proteins, especially in hepatoma cells. Thus, it may be considered a potential therapeutic agent for the treatment of hepatoma.

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References

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Published

2023-06-29

How to Cite

1.
Szaefer H, Hadryś K, Gajewska H, Migdałek K, Krajka-Kuźniak V. Comparison of the effect of betanin on STAT3, STAT5, and KAP1 proteins in HepG2 and THLE-2 cells. JMS [Internet]. 2023 Jun. 29 [cited 2024 Jun. 16];92(2):e805. Available from: https://jms.ump.edu.pl/index.php/JMS/article/view/805

Issue

Section

Original Papers
Received 2023-01-16
Accepted 2023-04-03
Published 2023-06-29