Histological disorders of neurons of phylogenetically different parts of the cerebral cortex in partial, subtotal, stepwise subtotal, and total cerebral ischemia

Authors

DOI:

https://doi.org/10.20883/medical.e493

Keywords:

cerebral ischemia, parietal cortex, neurons, rats, hippocampus

Abstract

Aim. Measure of the histological changes in neurons in the parietal cortex and hippocampus of rats with partial, subtotal, stepwise subtotal, and total cerebral ischemia.

Material and Methods. Studies were performed on 84 rats. Partial cerebral ischemia was modelled by ligation of one common carotid artery. Subtotal cerebral ischemia was modelled by ligation of both common carotid arteries. Stepwise subtotal cerebral ischemia was performed by sequential ligation of both common carotid artery with 7-day, 3-day or 1-day intervals. Total cerebral ischemia (CI) was modelled by decapitation.

Results. When comparing the morphological changes of neurons in the parietal cortex and hippocampus, we observed that, with the aggravation of the severity of cerebral ischemia, there was a progressive increase in the number of hyperchromic shrivelled neurons and neurons with pericellular oedema. Modelling of more severe types of ischemic damage lead to pronounced morphological changes in neurons – a decrease in size, deformation of the perikaryon, and increase in the degree of neuronal chromatophilia with their wrinkling.

Conclusions. The smallest morphological changes in neurons were noted in the partial cerebral ischemia groups and subgroup 1 of stepwise subtotal cerebral ischemia, with an interval between common carotid artery dressings of 7 days. The most obvious morphological changes were observed in the conditions of total cerebral ischemia after 1 day. Changes in the parietal cortex and hippocampus were unidirectional, but in the parietal cortex, which is most sensitive to oxygen deficiency, they were more pronounced.

Downloads

Download data is not yet available.

Author Biographies

Lizaveta I. Bon, Grodno State Medical University, Grodno, Belarus

Lizaveta I. Bon
Candidate of biological science, assistant professor of Pathophysiology Department named after D.A. Maslakov, Grodno State Medical University
е-mail: asphodela@list.ru
Grodno State Medical University, 80, Gorky St., 230009, Grodno, Belarus
Phone: +375336878764

 

Nataliya Y. Maksimovich, Department of Pathological Physiology named after D.A. Maslakov, Grodno State Medical University, Grodno, Belarus

Doctor of Medical Science, professor, head of the Department of Pathological Physiology named after D.A. Maslakov, Grodno State Medical University

References

Sveinsson ÓÁ, Kjartansson Ó, Valdimarsson EM. Heilablóðþurrð / heiladrep. Faraldsfræði, orsakir og einkenni. Læknablaðið. 2014 May 2;2014(05):271-279. https://doi.org/10.17992/lbl.2014.05.543

Qian H, Zhang H, Yin L, Zhang J. Postischemic Housing Environment on Cerebral Metabolism and Neuron Apoptosis after Focal Cerebral Ischemia in Rats. Current Medical Science. 2018 Aug;38(4):656-665. https://doi.org/10.1007/s11596-018-1927-9

Rahaman P, Del Bigio MR. Histology of Brain Trauma and Hypoxia-Ischemia. Academic Forensic Pathology. 2018 Aug 31;8(3):539-554. https://doi.org/10.1177/1925362118797728

Zhang L, Zhang RL, Jiang Q, Ding G, Chopp M, Zhang ZG. Focal embolic cerebral ischemia in the rat. Nature Protocols. 2015 Mar 5;10(4):539-547. https://doi.org/10.1038/nprot.2015.036

Igorevna Bon L. Effects of Experemental Cerebral Ishemia on Metabolic Characteristics of Parietal Cortex Neurons. Bioprocess Engineering. 2018;2(1):1. https://doi.org/10.11648/j.be.20180201.11

Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. Australia: Academic Press; 1998.

Bon L, Maksimovich N, Zimatkin S. Changes in the immunoreactivity of ATP synthase and chromatophilia of the cytoplasm of neurons in the parietal cortex of rats under conditions of stepwise cerebral ischemia. Orenburg Medical Bulletin. 2020;4:26-31.

Maksimovich N, Bon L, Dremza I. Structural and functional features of mitochondria and methods of their study in experiment. Medicus. 2019;5:8-18.

Zimatkin SM, Bon’ EI. Dark Neurons of the Brain. Neuroscience and Behavioral Physiology. 2018 Oct;48(8):908-912. https://doi.org/10.1007/s11055-018-0648-7

Kherani ZS, Auer RN. Pharmacologic analysis of the mechanism of dark neuron production in cerebral cortex. Acta Neuropathologica. 2008 Jun 3;116(4):447-452. https://doi.org/10.1007/s00401-008-0386-y

Capo I, Lalosević D. [Interpretation of dark neurons in experimental model of ischemia, neurointoxication and brain infection]. Med Pregl. 2011 Jan-Feb;64(1-2):101-6. PMID 21548279

Gallyas F, Pál J, Bukovics P. Supravital microwave experiments support that the formation of “dark” neurons is propelled by phase transition in an intracellular gel system. Brain Research. 2009 May;1270:152-156. https://doi.org/10.1016/j.brainres.2009.03.020

Morphological disturbances of rat parietal cortex and hippocampus neurons in the dynamics steady subtotal ischemia of the brain. Сибирский научный медицинский журнал. 2020;(2). https://doi.org/10.15372/ssmj20200204

Bon LI, Maksimovich NYe, Zimatkin SM. Morphological disorders of neurons in the hippocampus of rats with subtotal and total ischemia. Orenburg Medical Bulletin. 2020;2:41-6.

Downloads

Published

2021-03-24

How to Cite

1.
Bon LI, Maksimovich NY. Histological disorders of neurons of phylogenetically different parts of the cerebral cortex in partial, subtotal, stepwise subtotal, and total cerebral ischemia. JMS [Internet]. 2021 Mar. 24 [cited 2024 Jun. 16];90(1):e493. Available from: https://jms.ump.edu.pl/index.php/JMS/article/view/493

Issue

Section

Original Papers
Received 2021-01-04
Accepted 2021-03-11
Published 2021-03-24