Morphology of rat brain neurons in subtotal ischaemia and introduction of L-NAME and omega-3 polyunsaturated fatty acids

Authors

DOI:

https://doi.org/10.20883/medical.e423

Keywords:

cerebral ischaemia, parietal cortex, hippocampus, omega-3 polyunsaturated fatty acids, Nω-nitro-L-Arginine Methyl Ester

Abstract

Introduction. Cerebral ischaemia leads to the development of numerous morphofunctional disorders of the cerebral cortex, which can be exacerbated by the introduction of a Nω-nitro-L-arginine methyl ester (L-NAME), which is a non-selective inhibitor of nitric oxide synthase (NOS).

Aim. To study the morphological features of rat brain neurons in subtotal ischaemia during the administration of L-NAME and naturally occurring omega-3 polyunsaturated docosahexaenoic acid (DHA).

Material and Methods. Subtotal cerebral ischaemia was modelled in rats by ligation of both common carotid arteries. L-NAME and DHA were given individually or in combination to separate groups of rats. L-NAME (5 mg/kg) was administered intramuscularly immediately before ligation and DHA (5 mg/kg) intragastrically during the week before ligation.

Results. The introduction of DHA alone had a corrective effect on the hippocampus under conditions of subtotal ischaemia, reducing the number of shadow cells and hyperchromic wrinkled neurons, without significantly affecting the size and shape of the neurons of the parietal cortex. However, the previous administration of DHA to rats with cerebral ischaemia receiving a NOS inhibitor did not abrogate the negative effects on the state of the neurons in the cerebral cortex.

Conclusion. The administration of DHA can modulate the morphological disorder of the hippocampus which occurs in subtotal cerebral ischaemia.

Downloads

Download data is not yet available.

References

Maksimovich NY, Pronko TP, Maksimovich YN, Yermak VV. Epidemiology of ischemic strokes in the Grodno region (Belarus). Cerebrovascular Diesases. 2015 Jan 170-170;39.

Igorevna Bon L. Effects of Experemental Cerebral Ishemia on Metabolic Characteristics of Parietal Cortex Neurons. Bioprocess Engineering. 2018;2(1):1. https://doi.org/10.11648/j.be.20180201.11

Maksimovich N, Zinchuk V, Maslacov D. The degree of oxidative stress in the rat brain during ischemia and reperfusion in conditions of correction of the L-arginine-NO system. Neuroscience and Behavioral Physiol. 2006;36(4):373-8.

Maksimovich N. Tolerance of hypoxic hypoxia in rats with cerebral ischaemia treated by NO-synthase modulators. Hypoxia Medical. 2004;V(1-2):20-3.

Kaliannan K, Li X, Wang B, Pan Q, Chen C, Hao L, Xie S, Kang JX. Multi-omic analysis in transgenic mice implicates omega-6/omega-3 fatty acid imbalance as a risk factor for chronic disease. Communications Biology. 2019 Jul 26;2(1). https://doi.org/10.1038/s42003-019-0521-4

Khunt D, Shrivas M, Polaka S, Gondaliya P, Misra M. Role of Omega-3 Fatty Acids and Butter Oil in Targeting Delivery of Donepezil Hydrochloride Microemulsion to Brain via the Intranasal Route: a Comparative Study. AAPS PharmSciTech. 2020 Jan 3;21(2). https://doi.org/10.1208/s12249-019-1585-7

Kangari H, Eftekhari MH, Sardari S, Hashemi H, Salamzadeh J, Ghassemi-Broumand M, Khabazkhoob M. Short-term Consumption of Oral Omega-3 and Dry Eye Syndrome. Ophthalmology. 2013 Nov;120(11):2191-2196. https://doi.org/10.1016/j.ophtha.2013.04.006

Wu B, Song Q, Zhang Y, Wang C, Yang M, Zhang J, Han W, Jiang P. Antidepressant activity of ω-3 polyunsaturated fatty acids in ovariectomized rats: role of neuroinflammation and microglial polarization. Lipids in Health and Disease. 2020 Jan 8;19(1). https://doi.org/10.1186/s12944-020-1185-2

Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. 6. Australia: Academic Press; 1998.

Gallyas F, Pál J, Bukovics P. Supravital microwave experiments support that the formation of “dark” neurons is propelled by phase transition in an intracellular gel system. Brain Research. 2009 May;1270:152-156. https://doi.org/10.1016/j.brainres.2009.03.020

Zimatkin SM, Bon EI. Dark Neurons of the Brain. Neuroscience and Behavioral Physiology. 2018 Oct;48(8):908-912. https://doi.org/10.1007/s11055-018-0648-7

Shahidi F, Ambigaipalan P. Omega-3 Polyunsaturated Fatty Acids and Their Health Benefits. Annual Review of Food Science and Technology. 2018 Mar 25;9(1):345-381. https://doi.org/10.1146/annurev-food-111317-095850

Grosso G, Galvano F, Marventano S, Malaguarnera M, Bucolo C, Drago F, Caraci F. Omega-3 Fatty Acids and Depression: Scientific Evidence and Biological Mechanisms. Oxidative Medicine and Cellular Longevity. 2014;2014:1-16. https://doi.org/10.1155/2014/313570

Serini S, Calviello G. Long-chain omega-3 fatty acids and cancer. Current Opinion in Clinical Nutrition & Metabolic Care. 2018 Mar;21(2):83-89. https://doi.org/10.1097/mco.0000000000000439

Rogers T, Seehusen D. Omega-3 Fatty Acids and Cardiovascular Disease. Am Fam Physician. 2018;97(9):562-4.

Downloads

Published

2020-09-30

Issue

Section

Original Papers

How to Cite

1.
Bon L, Maksimovich NY, Zimatkin SM. Morphology of rat brain neurons in subtotal ischaemia and introduction of L-NAME and omega-3 polyunsaturated fatty acids. JMS [Internet]. 2020 Sep. 30 [cited 2024 Nov. 24];89(3):e423. Available from: https://jms.ump.edu.pl/index.php/JMS/article/view/423
Received 2020-04-16
Accepted 2020-09-18
Published 2020-09-30