Leucocytes and Th-associated cytokine profile of HIV-leishmaniasis coinfected patients attending the Abuja Teaching Hospital, Nigeria

Authors

  • Idris Nasir Abdullahi Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University, Zaria, Nigeria https://orcid.org/0000-0002-5511-1272
  • Anthony Uchenna Emeribe Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria
  • Hafeez Aderinsayo Adekola Department of Microbiology, Olabisi Onabanjo University, Ogun state, Nigeria
  • Habiba Yahaya Muhammad Department of Medical Laboratory Science, Bayero University Kano, Nigeria
  • Abdurrahman El-fulaty Ahmad Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University Zaria Nigeria
  • Abubakar Umar Anka Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University Zaria Nigeria
  • Shamsuddeen Haruna Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University Zaria Nigeria
  • Bamidele Soji Oderinde Department of Medical Laboratory Science, University of Maiduguri, Maiduguri, Nigeria https://orcid.org/0000-0003-4420-8607
  • Yusuf Mohammed Department of Medical Microbiology and Parasitology, Bayero University Kano, Nigeria
  • Halima Ali Shuwa Department of Community Health, Federal University Dutse, Jigawa State, Nigeria
  • Adamu Babayo Department of Medical Microbiology and Parasitology, Bayero University Kano, Nigeria

DOI:

https://doi.org/10.20883/medical.408

Keywords:

Cellular Immunity, Cytokines, Leishmaniasis, Pro-inflammation, HIV co-infection

Abstract

Introduction. T-helper cells (Th)-1& -2 cytokines homeostasis control or predict clinical outcome of Leishmania spp infected persons, especially those living with HIV /AIDS. This case-control study evaluated the leucocytes differentials, TNF-alpha, interleukin (IL)-2 and -10 levels among HIV infected persons with serological evidence of leishmaniasis attending University of Abuja Teaching Hospital, Nigeria

Material and Methods. Blood samples from 28 HIV infected persons who were Leishmania donovani rK39 and IgG positive (group 1); 30 age- & -sex matched HIV infected persons without Leishmania antibodies (group 2); and 30 apparently healthy persons without HIV and Leishmania donovani antibodies (group 3). Full blood counts, TNF alpha, IL-2 and -10 levels were analyzed using automated hematology analyzer and ELISA, respectively. Structured questionnaires were used to collate biodata and clinical presentations of participants.

Results. Ten (35.7%) participants in group 1 were on ART, 15 (50%) in group 2 were on ART, while group 3 were ART naïve. There were significantly higher values in basophil (4.4 ± 2.5%) and eosinophil counts (12.9 ± 3.8%) in HIV/leishmania coinfected persons (p˂0.005). However, other white cells subpopulation was significantly lower in HIV/leishmania co-infected persons (p˂ 0.05). There was significantly reduced CD4+ T cell counts ([ 119±26 versus 348±63 versus 605±116 cells/mm3]) , TNF-alpha ([ 36.82±8.21 versus 64.67±12.54 versus 254.98±65.59pg/mL]) and IL-2 levels ([ 142.14±20.91 versus 507.6±84.42versus 486.62±167.87 pg/mL]) among HIV/Leishmania co-infected persons compared to group 2 and group 3 participants, respectively. However, higher IL-10 level (80.35±14.57pg/mL) was found in HIV/Leishmania co-infected persons as opposed to the HIV mono-infected (62.2±10.43pg/mL) and apparently healthy persons (23.97±4.88pg/mL) (p˂0.001).

Conclusions. Eosinophil, basophil counts and serum IL-10 level were high in HIV/Leishmania coinfected persons, demonstrating Leishmania -induced hypersensitivity and immunosuppression.

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Published

2020-01-30

How to Cite

1.
Abdullahi IN, Emeribe AU, Adekola HA, Muhammad HY, Ahmad AE- fulaty, Anka AU, Haruna S, Oderinde BS, Mohammed Y, Shuwa HA, Babayo A. Leucocytes and Th-associated cytokine profile of HIV-leishmaniasis coinfected patients attending the Abuja Teaching Hospital, Nigeria. JMS [Internet]. 2020Jan.30 [cited 2020May25];89(1):e408. Available from: https://jms.ump.edu.pl/index.php/JMS/article/view/408

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Section

Original Papers