Monitoring the skin NADH changes during ischaemia and reperfusion in humans




460-nm fluorescence, ischaemia, microcirculation, mitochondria, nicotinamide adenine dinucleotide, reperfusion


Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in many important biochemical reactions in human metabolism, including participation in energy production by mitochondria. Flow Mediated Skin Fluorescence (FMSF) is a non-invasive method to study dynamic changes in the content of the reduced form of NADH by measuring the optical properties of NADH related to the emission of the autofluorescent light (460 nm) after an earlier excitation by ultraviolet light. This review summarises the available studies using this method to describe its potential and limitations.


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How to Cite

Niziński J, Kamieniarz L, Filberek P, Sibrecht G, Guzik P. Monitoring the skin NADH changes during ischaemia and reperfusion in humans. JMS [Internet]. 2020 Mar. 31 [cited 2024 Jun. 18];89(1):e405. Available from:



Review Papers
Received 2020-01-25
Accepted 2020-04-01
Published 2020-03-31