Rescue from bone loss, diminished mineral density and strength in mice after treatment with steroid and nonsteroid anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs.

Anti-inflammatory drugs impact on bones

  • Tomasz P. Lehmann Department of Biochemistry and Molecular Biology
  • Ewa Pruszyńska-Oszmałek
  • Paweł Kołodziejski
  • Magdalena Wojtków
  • Celina Pezowicz
  • Mirosław Szybowicz
  • Paweł P. Jagodziński
  • Marek Nowicki
  • Aleksandra Trzaskowska
  • Sławomir Mielcarek
  • Maciej Głowacki
Keywords: methylprednisolone, diclofenac, miRNA, bone, exercise, bone strenght

Abstract

The project “Rescue from bone loss, diminished mineral density and strength in mice after treatment with steroid and nonsteroid anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs” is an animal experiment project, and an attempt to apply small RNA to reverse harmful effects of anti-inflammatory drugs. Patients chronically treated with anti-inflammatory drug suffer from musculo-skeletal side effects, including reversing the mineralization and disabling bone fracture healing. Aims of the study will be to measure changes in bone mineral density and bone strength in mice treated with methylprednisolone or diclofenac in combination with treadmill exercise. The reversal of the negative effects of drugs will be assayed using modified miRNA agomir. Bones obtained from treated mice will be analyzed using micro-CT, dynamic mechanical analysis (DMA), nanoindentation, Raman spectroscopy and gene expression. We expect to find specific miRNA counteracting demineralization caused by methylprednisolone or diclofenac of the mice bones.