Recovery from bone loss, diminished mineral density and strength in mice after treatment with steroidal and nonsteroidal anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs: Anti-inflammatory drugs impact on bones

Tomasz P. Lehmann; Ewa Pruszyńska-Oszmałek; Paweł Kołodziejski; Magdalena Wojtków; Celina Pezowicz; Mirosław Szybowicz; Paweł P. Jagodziński; Marek Nowicki; Aleksandra Trzaskowska; Sławomir Mielcarek; Maciej Głowacki

doi:10.20883/medical.379

Authors

Tomasz P. Lehmann Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poland https://orcid.org/0000-0001-8445-0970
Ewa Pruszyńska-Oszmałek Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poland https://orcid.org/0000-0002-7182-6905
Paweł Kołodziejski Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poland https://orcid.org/0000-0002-7941-0955
Magdalena Wojtków Department of Biomedical Engineering, Mechatronics and Theory of Mechanisms, Wroclaw University of Science and Technology, Poland https://orcid.org/0000-0003-0082-0386
Celina Pezowicz Department of Biomedical Engineering, Mechatronics and Theory of Mechanisms, Wroclaw University of Science and Technology, Poland https://orcid.org/0000-0002-3516-4764
Mirosław Szybowicz Institute of Materials Research and Quantum Engineering, Faculty of Technical Physics, Poznan University of Technology, Poland https://orcid.org/0000-0001-8933-571X
Paweł P. Jagodziński Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poland https://orcid.org/0000-0002-9046-6802
Marek Nowicki Wielkopolska Centre for Advanced Technologies, Poznań, Poland https://orcid.org/0000-0002-0305-6881
Aleksandra Trzaskowska Crystal Physics Division, Faculty of Physics, Adam Mickiewicz University, Poznań, Poland https://orcid.org/0000-0003-0451-7093
Sławomir Mielcarek Crystal Physics Division, Faculty of Physics, Adam Mickiewicz University, Poznań, Poland https://orcid.org/0000-0002-0215-3313
Maciej Głowacki Department of Paediatric Orthopaedics and Traumatology, Poznan University of Medical Sciences, Poland https://orcid.org/0000-0002-9932-670X

DOI:

https://doi.org/10.20883/medical.379

Keywords:

methylprednisolone, diclofenac, miRNA, bone, exercise, bone strenght

Abstract

The project “Recovery from bone loss, diminished mineral density and strength in mice after treatment with steroidal and nonsteroidal anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs” is an animal experiment project, and an attempt to apply small RNA strands to reverse the harmful effects of anti-inflammatory drugs. Patients chronically treated with an anti-inflammatory drug suffer from musculoskeletal side effects, including reversed mineralisation and disabled bone fracture healing. The aims of the study are to measure changes in bone mineral density and bone strength in mice treated with methylprednisolone or diclofenac in combination with treadmill exercise. The reversal of the negative effects of these drugs will be assayed using modified miRNA agomir. Bones obtained from the treated mice will be analysed using micro-CT, dynamic mechanical analysis (DMA), nanoindentation, Raman spectroscopy and gene expression. We expect to find specific miRNA counteracting the demineralisation of the mice bones caused by methylprednisolone or diclofenac.

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