Immunohistochemical evaluation of cellular composition of the immune system of lymph nodes in acute appendicitis

Main Article Content

Jakub Żurawski
Patrycja Talarska
Stanisław Łazowski
Marcin Grochowalski
Jacek Karoń

Abstract

Introduction. There is not much data about the composition of populations of the immune system in acute appendicitis. The basic histopathological criterion for the diagnosis of acute appendicitis is neutrophil infiltration of the muscle membrane.
Aim. The subject of this publication is a semi-quantitative evaluation of B lymphocytes (CD20+), T lymphocytes (CD3+) and macrophages (CD68+), and the determination of the number of active lymph nodes during the course of inflammation.
Material and Methods. The study material was obtained from 79 patients who had an appendectomy due to acute appendicitis. In this group, the tissue was obtained from: 34 women (aged 20 to 91) and 45 men (aged 20 to 72).
Results. In the course of acute appendicitis, there is involvement of lymph node B lymphocytes, T lymphocytes and macrophages. Independent of the type of inflammation, the cellular make-up of the nodes is similar. The number of lymph nodes decreases with age and is gender dependent.
Conclusions. In the course of acute appendicitis, there is involvement of lymph node B lymphocytes, T lymphocytes and macrophages. The number of lymph nodes decreases with age and is gender dependent. A statistically significant number of the examined cells of the immunological system in the lymph nodes changed due to inflammation (p<0.001). B and T lymphocytes in the lymph nodes and in the mucous membrane of the appendix differed depending on the sex, and the presence of B lymphocytes in the mucous membrane was significantly higher in the group of 20-40 years of age. T lymphocytes were predominant in the centres of the lymph nodes in groups 20-40 and 61-91 years of age, and in the peripheral zones in the group of 41-60 years of age.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Article Details

How to Cite
1.
Żurawski J, Talarska P, Łazowski S, Grochowalski M, Karoń J. Immunohistochemical evaluation of cellular composition of the immune system of lymph nodes in acute appendicitis. JMS [Internet]. 2019Dec.12 [cited 2020Jul.11];88(4):218-26. Available from: https://jms.ump.edu.pl/index.php/JMS/article/view/368
Section
Original Papers

References

Lamps LW. Infectious causes of appendicitis. Infect Dis Clin North Am. 2010;4:995–1018.

Kooij IA, Sahami S, Meijer SL, Buskens CJ, Te Velde AA. The immunology of the vermiform appendix: a review of the literature. Clin Exp Immunol. 2016;186:1–9.

Cartwright SL, Knudson MP. Evaluation of Acute Abdominal Pain in Adults. Am Fam Physician. 2008;77:971–978.

Herd ME, Cross PA, Dutt S. Histological audit of acute appendicitis. J Clin Pathol. 1992;45:456–458.

Benhamou G. Useless appendectomy, its diagnostic difficulties. Ann Gastroenterol Hepatol (Paris). 1986;22:339–340.

Güller U, Oertli D, Terracciano L, Harder F. Neurogenic appendicopathy: a frequent, almost unknown disease picture. Evaluation of 816 appendices and review of the literature. Chir Z Alle Geb Oper Medizen. 2001;72:684–689.

Di Sebastiano P, Fink T, di Mola FF, Weihe E, Innocenti P, Friess H, et al. Neuroimmune appendicitis. Lancet Lond Engl. 1999;354:461–466.

Gebbers JO, Laissue JA. Bacterial translocation in the normal human appendix parallels the development of the local immune system. Ann N Y Acad Sci. 2004;1029:337–343.

Guller U, Hervey S, Purves H, Muhlbaier LH, Peterson ED, Eubanks S, et al. Laparoscopic versus open appendectomy: outcomes comparison based on a large administrative database. Ann Surg. 2004;239:43–52.