Immunohistochemical evaluation of cellular composition of the immune system of lymph nodes in acute appendicitis

  • Jakub Żurawski Chair of Biology and Environmental Sciences, Department of Immunobiochemistry Poznan University of Medical Sciences, Poland
  • Patrycja Talarska
  • Stanisław Łazowski
  • Marcin Grochowalski
  • Jacek Karoń
Keywords: appendicitis, lymph nodes, histomorphometry

Abstract

Introduction: There is not much data about the composition of populations of the immune system in acute appendicites. The basic histopathological criterion for the diagnosis of acute appendicitis is the neutrophil infiltration in the muscle membrane.The subject of this publication is semi-quantitative evaluation of B lymphocytes (CD20+), T lymphocytes (CD3+) and macrophages (CD68+), and determination of the number of active lymph nodes during the course of inflammation. Material and methods: The study material was obtained from 79 patients who had appendectomy due to acute appendicitis. In this group, the tissue was obtained from: 34 women (aged 20 to 91) and 45 men (aged 20 to 72). Results: In the course of acute appendicitis, there is an involvement of lymph node B lymphocytes, T lymphocytes and macrophages. Independently of the type of inflammation, the cellular make up of nodes is similar. The number of lymph nodes decreases with age and is gender dependent. Conclusions: In the course of acute appendicitis there is an involvement of lymph node  B lymphocytes, T lymphocytes and macrophages. The number of lymph nodes decreases with age and is gender dependent. A statistically significant difference in the number of examined cells of the immunological system of lymph nodes changed due to inflammation (p<0.001) was observed. B and T lymphocytes in lymph nodes and in the mucous membrane of the appendix differed depending on the sex. Presence of B lymphocytes in the mucous membrane was significantly higher in the group of 20-40 years of age. T lymphocytes were predominant in the centre of lymph nodes in groups 20-40 and 61-91 years of age, and in the peripheral zone in the group of 41-60 years of age.