Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients

Anna Olewicz-Gawlik; Izabela Korczowska-Łącka; Paweł Hrycaj

doi:10.20883/medical.e33

Authors

Anna Olewicz-Gawlik Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Poland
Izabela Korczowska-Łącka Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Poland
Paweł Hrycaj Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Poland

DOI:

https://doi.org/10.20883/medical.e33

Keywords:

acute phase proteins, fucosylation, rheumatoid arthritis, selectins

Abstract

Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation.
Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients.
Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA).
Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p < 0.001) and significantly correlated with serum concentrations of rheumatoid factor (RF) and antibodies against cyclic citrullinated peptides (ACPA) (p = 0.006, p = 0.04, respectively). Moreover, we found significant correlations between the serum levels of soluble (s)P- and sE-selectin and ACT-FR (p = 0.008 and p = 0.03, respectively) only in male RA patients.
Conclusions. Fucosylation of ACT differs between male and female RA patients and is related to sP- and sE-selectin levels only in men.

Downloads

Download data is not yet available.

References

Lee DM, Weinblatt ME. Rheumatoid arthritis. Lancet 2001 Sep; 358(9285):903–11.

Alamanos Y, Drosos AA. Epidemiology of adult rheumatoid arthritis. Autoimmun Rev 2005 Mar; 4(3):130–36.

Tedder TF, Steeber DA, Chen A, Engel P. The selectins: vascular adhesion molecules. FASEB J 1995 Jul; 9(10):866–73.

Osborn L. Leukocyte adhesion to endothelium in inflammation. Cell 1990 Jul; 62(1):3–6.

Eppihimer MJ, Wolitzky B, Anderson DC, Labow MA, Granger DN. Heterogeneity of expression of E- and P-selectins in vivo. Circ Res. 1996 Sep;79(3):560–9.

Koch AE, Halloran MM, Haskell CJ, Shah MR, Polverini PJ: Angiogenesis mediated by soluble forms of E-selectin and vascular cell adhesion molecule-1. Nature 1995 Aug; 376(6540):517–19.

Schleiffenbaum B, Spertini O, Tedder TF. Soluble L-selectin is present in human plasma at high levels and retains functional activity. J Cell Biol. 1992 Oct;119(1):229–38.

Ates A, Kinikli G, Turgay M, Duman M. Serum-soluble selectin levels in patients with rheumatoid arthritis and systemic sclerosis. Scand J Immunol 2004 Mar; 59(3):315–20.

Foxall C, Watson SR, Dowbenko D et al. The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide. J Cell Biol 1992 May; 117(4):895–902.

Rombouts Y, Ewing E, van de Stadt LA et al. Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis. Ann Rheum Dis. 2015 Jan;74(1):234–41.

Roy S, Biswas S, Saroha A, Sahu D, Das HR. Enhanced expression and fucosylation of ficolin3 in plasma of RA patients. Clin Biochem. 2013 Jan;46(1–2):160–3.

Brinkman-van der Linden EC, de Haan PF, Havenaar EC, van Dijk W. Inflammation-induced expression of sialyl LewisX is not restricted to ?1-acid glycoprotein but also occurs to a lesser extent on ?1-antichymotrypsin and haptoglobin. Glycoconj J 1998 Feb;15(2):177–82.

Baker C, Belbin O, Kalsheker N, Morgan K. SERPINA3 (alpha-1-antichymotrypsin). Front Biosci. 2007 May;12:2821–35.

Chard MD, Calvin J, Price CP, Cawston TE, Hazleman BL. Serum alpha 1 antichymotrypsin concentration as a marker of disease activity in rheumatoid arthritis. Ann Rheum Dis. 1988 Aug;47(8):665–71.

Miyata J, Tani K, Sato K et al. Cathepsin G: the significance in rheumatoid arthritis as a monocyte chemoattractant. Rheumatol Int. 2007 Feb;27(4):375–82.

Naidoo N, Cooperman BS, Wang ZM, Liu XZ, Rubin H. Identification of lysines within alpha 1-antichymotrypsin important for DNA binding. An unusual combination of DNA-binding elements. J Biol Chem 1995 Jun; 270(24):14548–55.

Aletaha D, Neogi T, Silman AJ et al. 2010 Rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative. Arthritis Rheum 2010 Sep; 62(9):2569–81.

Van der Heijde DM, van 't Hof M, van Riel PL, van de Putte LB. Development of a disease activity score based on judgment in clinical practice by rheumatologists. J Rheumatol 1993 Mar; 20(3):579–81.

Prevoo ML, van 't Hof MA, Kuper HH, van Leeuwen MA, van de Putte LB, van Riel PL. Modified disease activity scores that include twenty-eight-joint counts. Development and validation in a prospective longitudinal study of patients with rheumatoid arthritis. Arthritis Rheum 1995 Jan; 38(1):44–48.

Fries JF, Spitz P, Kraines RG, Holman HR. Measurement of patient outcome in arthritis. Arthritis Rheum 1980 Feb; 23(2):137–45.

Bird HA, Dixon JS. The measurement of pain. Baillieres Clin Rheumatol 1987 Apr; 1(1):71–89.

Laurell CB. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry 1966 Apr; 15(1):45–52.

Ryden I, Lundblad A, Pahlsson P. Lectin ELISA for analysis of ?(1)-acid glycoprotein fucosylation in the acute phase response. Clin Chem 1999 Nov; 45(11):2010–12.

Brinkman-Van der Linden CM, Havenaar EC, Van Ommen CR, Van Kamp GJ, Gooren LJ, Van Dijk W. Oral estrogen treatment induces a decrease in expression of sialyl Lewis x on ? 1-acid glycoprotein in females and male-to-female transsexuals. Glycobiology 1996 Jun; 6(4):407–412.

Forslind K, Hafström I, Ahlmén M, Svensson B, the BARFOT Study Group. Sex: a major predictor of remission in early rheumatoid arthritis? Ann Rheum Dis 2007 Jan; 66(1):46–52.

Tengstrand B, Ahlmén M, Hafström I. The influence of sex on rheumatoid arthritis: a prospective study of onset and outcome after 2 years. J Rheumatol 2004 Feb; 31(2):214–222.

Ryden I, Pahlsson P, Lundblad A, Skogh T. Fucosylation of ?1-acid glycoprotein (orosomucoid) compared with traditional biochemical markers of inflammation in recent onset rheumatoid arthritis. Clin Chim Acta 2002 Mar; 317(1–2):221–29.

Miriovsky BJ, Michaud K, Thiele GM, et al. Anti-CCP antibody and rheumatoid factor concentrations predict greater disease activity in men with rheumatoid arthritis. Ann Rheum Dis. 2010 Jul;69(7):1292–7.